The unmutated form of your protein has a K m of between 53 and 54 M and

The unmutated form of your protein has a K_m of between 53 and 54 µM and a V_max of 43 mM/s. The enzyme kinetic data for your enzyme with the amino acid substitution should now be displayed in the table you produced. Based on these data, explain the effect of amino acid substitution on the K_m and V_max for the mutated protein.

Substrate concentration [S] uM	Initial velocity Vo s-1
10	0.25
25	0.45
50	0.62
100	0.76
150	0.81
200	0.85
300	0.90
500	0.93

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Solution A mutation in the amino acid sequence of th... View the full answer