Question: there are 20 sites from which study samples (Water samples from the sea) were collected and 5 species of aquatic vertebrates with which this research

there are 20 sites from which study samples (Water samples from

there are 20 sites from which study samples (Water samples from the sea) were collected and 5 species of aquatic vertebrates with which this research is interested, then: In terms of statistics, which analysis should be used to determine if the locations differ in the presence of the 5 species? and could you please explain the mechanics of this analysis in detail with an example? Thank you

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