Question: There are different types of reporter assays/systems. Examples include CAT, beta galactosidase, SEAP, luciferase, GFP, FlAsH/ReAsh and beta lactamase. Based on these examples: 1. If

There are different types of reporter assays/systems. Examples include CAT, beta galactosidase, SEAP, luciferase, GFP, FlAsH/ReAsh and beta lactamase. Based on these examples:

1. If I wanted to study interaction between protein A and protein B in cell-based assay, which of the reporter system should I use and why, how should I set up the assay and types of controls I would require?

2. If I wanted to test if my compound is activating NF-kB signaling, which reporter system should I use and why, how should I set up the assay and types of controls I would require?

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Biology Questions!

Grow It All Farms Ltd. is an agri-business that grows a variety of different grains on the land that it owns. Operations have been steadily expanding over the last few years. The company has now...

Question:DEPARTMENT OF SUPPLY CHAIN & INFORMATION SYSTEMS MANAGEMENT INFORMATION SYSTEMS & EBUSINESS Question 1. Perform a critique on the following journal article: Schlagwein, Daniel and Hu, Monica...

Read the attached journal article (Stoshkj 2014). :4 Marina Stoshikj this type of situations is that not always the involved parties are able to recognize that they are after different things and...

# Assignment Task # A- P1 : Explain sources and suitability of finance available in different business contexts ? A- P2: Explain sources and suitability of revenue available in a specific business...

Types of Innovations To remain competitive in an ever-changing marketplace, companies must introduce new products or modify their current products to keep customers satisfied and to meet competitor's...

Topic: Conducting personal job interviews using the star model 1-Design a two-hour training work plan for 10 trainees 2-Determine the quality of trainees 3-Use the training design model Formulate one...

Hello, I have a case study need be answeredregarding pdf files questions, 1.Research the capacity of municipalities in Bosnia and Herzegovina for issuing a bonds? 2.What are pros and cons of bond...

(1) Financial Ratios Referencing this week?s readings and lecture, what are the limitations of financial ratios? Classify your answer into at least the following categories: liquidity ratios,...

answer each and every part in detail because this assignment we are going to send uni of london please answer it wisely its finance question . That operate in different sectors (eg, retail,...

Hello, I have a case study need be answeredregarding pdf files questions, 1.Research the capacity of municipalities in Bosnia and Herzegovina for issuing a bonds? 2.What are pros and cons of bond...

Discuss the merits of a 50 percent hedging ratio.

Prove the following comparison theorems for real functions f and g, and a R. a) If f(JC) > g(x) and g(x) as x a, then f(x) as x a. b) If f(x) then g(x) L as . L:= lim f(x) = lim h(x).

A nurse is assessing a client who is receiving epoetin alfa to treat anemia. Which of the following findings should the nurse monitor? . O o O O o . . . o . O Paresthesia Increased blood pressure...

Julian Birkinshaw, James Manktelow, Vittorio DAmato, Elena Tosca, and Francesca Macchi In recent years, one of the most profound transformations in the workplace has been the increase in age...