Question: There is a substance in a solution (5 g/liter). The length of cuvette is 1 cm and only 25% (I = 0.25 I0) of the
There is a substance in a solution (5 g/liter). The length of cuvette is 1 cm and only 25% (I = 0.25 I0) of the certain light beam is transmitted.
a) What is the specific extinction coefficient ?
b) What is the molar specific extinction coefficient if the molecular weight is 80 g/mol ?
c) If the absorbance of an another sample of the same substance is 0.8 (the length of cuvette is 1 cm) what is the concentration of the sample ?
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