Thymidylate synthase (TS) is critical in the early stages of DNA biosynthesis. Thymidylate synthase (show below) possesses three critical amino acid residues: Cys 198, Arg 218, and Glu 60. What are the critical interactions shown for each amino acid shown below. Propose mutations that would not result in a loss of activity for each critical amino acid.

Transcribed Image Text:

E-Glu60- OH deoxyR H₂N E-Cys198 E-Glu60- NH NH E-Arg218 deoxyR wwwwwwwww S E-Cys198 IZ + MTF CH2 NHR Thymidine E-Glu60- OH deoxyR H₂N E-Cys198 E-Glu60- NH NH E-Arg218 deoxyR wwwwwwwww S E-Cys198 IZ + MTF CH2 NHR Thymidine

Answer rating: 100% (QA)

Amino acid substitutions are usually performed to confirm the role of each amino acid in the structure or function of the protein Similarly treatment ... View the full answer