Tm $($No SOS$)$ Tm $($With SOS$)$

A $30\backslash$deg C $35\mathrm{.}5\backslash$deg C

B $40\backslash$deg C $43\mathrm{.}2\backslash$deg C

C $35\backslash$deg C $43\mathrm{.}2\backslash$deg C

$1)$ Rank the above hFGF$-1$ mutants in order of increasing thermal stability. Also rank them in order of increasing SOS binding.

$2)$ Rank the above hFGF$-1$ mutants in order of increasing SOS affinity.