Question: Tris is a monoprotic buffer commonly used in biochemical experiments. A buffer is prepared with 0.102 mol of the basic form of Tris. This amount

Tris is a monoprotic buffer commonly used in biochemical experiments. A buffer is prepared with 0.102 mol of the basic form of Tris. This amount of base represents a 90.3% mole fraction of the total buffer content. The total volume of buffer is 450 mL, and the final pH is 9.2. Calculate ( 1) the pKa of Tris to 3 decimal places, and (2) the total concentration of Tris in the final buffer to 3 decimal place

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!

7. How many mL of 0.2 M HCl should be added to 50 mL of 0.2 M Tris base (Trishydroxymethyl aminomethane) to make a buffer of pH 8.0? (Hint: working with concentrations and then ratios of mmoles of...

Proteases are active ingredients in laundry detergents for degradation of proteinaceous stains. In the last 5 0 years, proteases switched from being minor additives to key ingredients. Numerous...

of 1000 Hepl Protein Seguences. together, and then placed in a 80C freezer for 20min to cellulose, which is eesential because Hept binds sugarfreene the solation, followed by lyophilization to...

18. Tris is the basic form of a common biological buffer. What volume of 0.100 M HBr would have to be added to a 125 mL solution of 0.0250 M Tris to make a buffer with pH 7.5? (Hint: Work in moles,...

Tris is an organic buffer that is used a lot in biochemistry. It has a pKa of 8 . If you want to make a buffer with a pH of 7 out of it, what ratio of Tris-base to Tris-acid would you use? 10 5 1 0.1...

In gel electrophoresis, 1x tris acetate EDTA (TAE) buffer is required for running the gel. How much volume of 50x TAE is needed to prepare a 1000 mL solution to include a buffer for future use?...

Which term describes a substance that acts as a proton donor and a proton acceptor? Question 9 options: amphoteric polyprotic monoprotic buffer

Details of a mixture of three proteins are below. Protein A: 49,198 Da. pI= 4.71 Protein B: 15,273 Da. pI= 10.8 Protein C: 85,019 Da. pI= 7.26 a) At pH=8, which protein will elute first in an anion...

Chasity santos is in the process of computing net pay for the employees of Chastity Santos is in the process of computing net pay for the employees of Happy Crab Marketing. Place the following steps...

1. The following crew is normally capable of completing 22 squares (1 square=100 S.F.) per day of a built-up roofing systems composed of 3 plies of #15 asphalt felt, mopped asphalt flood coat, and...

A company issued $ 2 , 0 0 0 , 0 0 0 of 9 % bonds at face value on September 1 , 2 0 2 3 . The company elects to use the fair value option for these bonds. At December 3 1 , 2 0 2 3 , the value of...

Question 13 4 pts A 1-year zero-coupon Treasury bond has a promised yield of 3.5% and a 1-year zero-coupon corporate bond has a promised yield of 8.7%. The corporate bond has a default probability of...

identify the behaviour and results which are needed and define the rules which govern how individuals and groups behave;

be understood and accepted by employees as consistent with their values and priorities and be regarded as equitable and competitive.

provide rewards which are equitable, competitive, timely and in an appropriate form;