uote what I should annotate and for each annotation give me a one sentence comment I should say about the highlighted portion : Look over the slides from Week 1 "How to read a scientific article," and consider addressing these topics: 1) The major research question(s) explored. 2) What prior research was done? What research gaps does the author intend to fulfill? 3) What are their hypotheses? 4) How does the author address these gaps (i.e., methods)? 5) What's the main finding of each figure? 6) If you can choose, what is the ONE piece of data that was most important or directly addressing the question? 7) Do the author's interpretations match the evidence? (It's OK to disagree!) 8) Any critiques or further comments on the paper.

Sholl analysis of cell complexity Analysis was performed on O4* cells grown on poly- L-ornithine- and laminin-coated coverslips until day 76 of differentiation. After immunostaining for O04 (see above), cells were fixed with 4% PFA, labeled with DAPI, and coverslips mounted onto glass slides for imaging. Images were randomly acquired from 3 replicates/group at 20x magnification with a Zeiss Axiovert fluorescence microscope and a minimum of 25 cells/group were selected for morphological analysis, making sure that cells were sufficiently distanced to avoid process overlap. Sholl analysis of cell complexity was performed by a blinded investigator using Image J software. After manual tracing of individual cells, the number of \"nodes\" or \"process intersections' was automatically estimated at increasing distance from the cell center (4 zm increments). Total process length/cell was also quantified