use the table below to make a standard curve graph

Table of Absorbances for Bradford Assay:

Sample	Amount (?l)	Absorbance at 595 nm
Extract 1/10	20	0.838
Extract 1/100	20	0.604
Extract 1/1000	20	0.577
Blue Fraction (undiluted)	20	0.804
Blue Fraction 1/10	20	0.616
Blue Fraction 1/100	20	0.561
Yellow Fraction (undiluted)	20	0.567
Yellow Fraction 1/10	20	0.558
Yellow Fraction 1/100	20	0.547

using the table below make an Absorbance spectra of fractions

Table of Absorbances for Bradford Assay:

Sample	Amount (?l)	Absorbance at 595 nm
Protein Standard 0 mg/ml	20	0
Protein Standard 0.125 mg/ml	20	0.567
Protein Standard 0.25 mg/ml	20	0.601
Protein Standard 0.5 mg/ml	20	0.730
Protein Standard 0.75 mg/ml	20	0.854
Protein Standard 1.0 mg/ml	20	0.930
Protein Standard 1.5 mg/ml	20	1.374
Protein Standard 2.0 mg/ml	20
Extract 1/10	20	0.838
Extract 1/100	20	0.604
Extract 1/1000	20	0.577
Blue Fraction (undiluted)	20	0.804
Blue Fraction 1/10	20	0.616
Blue Fraction 1/100	20	0.561
Yellow Fraction (undiluted)	20	0.567
Yellow Fraction 1/10	20	0.558
Yellow Fraction 1/100	20	0.547

make a SDS-PAGE graph with calculations and molecular weights of purified proteins using the picture below

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