Question: Using a 20 cm long C18 bonded HPLC column filled with 5m size particles and a mobile phase consisting of 20% methanol in water, two

Using a 20 cm long C18 bonded HPLC column filled with 5m size particles and a mobile phase consisting of 20% methanol in water, two pesticides P1 and P2 elute at 9.0 and 9.2 minutes respectively with corresponding base peak widths of 30 and 30.7 seconds respectively. The mobile phase solvent peak elutes at 2 minutes. Calculate: the retention factors (k') for both pesticides the selectivity () the number of theoretical plates (N) and the Height Equivalent to a Theoretical Plate (H) the resolution Rs Identify the parameter (N, k', ) that is responsible for this low resolution and the practical step you would take to improve the separation.

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemical Engineering Questions!

4. Using a 20 cm long C18 bonded HPLC column filled with 5m size particles and a mobile phase consisting of 50% methanol in water, Vitamins B2 and B3 elute at 4.2 and 4.4 minutes respectively with...

Experiment 1: Ohm's Law In this experiment, you will apply Ohm's Law to investigate the relationship between voltage, resistance and current. Materials 4 AA Batteries 2 Alligator Clips Digital...

The experimental result differs from the assumption. The result shows that the ring oscillator frequencies can be grouped into two groups, where the first group is the high frequency group for the...

20. Scientists measured the lengths of a species of lizard in a region. The graph below shows that the measured lengths have a normal distribution. 10 12 14 16 18 20 22 24 26 28 30 32 34 Length (cm)...

An automotive catalytic convertor is a packed bed in which a platinum catalyst is coated on the surface of small alumina spheres. A metal container holds the catalyst pellets and allows engine...

An investigator has made a solenoid by wrapping 100 turns of wire on a tube that is 10 cm long and 2 cm in diameter. The power supply is providing as much current as it can, but a stronger field is...

B3. An 80 kg student is contemplating making a bungee jump from a platform 60 m above a canyon. Using an elastic bungee rope that is 30 m long when it is not loaded, and 35 m long when it supports...

Problem 1 : Based on the circuit diagram below, draw a phasor diagram to scale using 2 0 V / cm and 0 . 4 \ ( \ mathrm { A } / \ mathrm { cm } \ ) . If \ ( \ mathrm { V } _ { 1 } \ ) is represented...

Draw a phasor diagram to scale using 2 0 V / cm and 0 . 4 A / cm . If V 1 is represented by an arrow 1 . 6 cm long at an angle of - 9 0 degrees and V 2 by a 2 . 0 cm long arrow an undesignated angle...

Problem 1 : Based on the circuit diagram below, draw a phasor diagram to scale using 2 0 V c m and 0 . 4 A c m . If V 1 is represented by an arrow 1 . 6 cm long at an angle of - 9 0 degrees and V 2...

Consider the intertemporal budget constraint in equation (18.5). Assume the interest rate is I = 5%. (a) Suppose the government cuts taxes today by $100 billion. Describe three possible ways the...

The sector allocation of two funds and their benchmark was obtained on the 1st of July and are as follows: Sectors Financials Consumer cyclicals Consumer non-cyclicals Total Benchmark 49% 35% 16%...

All of the following are true of the Efficient Frontier for investments except: 1 0 - 3 0 year US Treasury bond yield helps define risk free return It is more appealing when investors can realize...

QUESTION 22 During the month of October Me D's Bicycle Store had the following transactions occured Oct Purchased 10 bicycles for $600 each on account, terms 2/10, 1/30 7 Purchases returns of $400...