Question: Using PCR technique, the two nucleotides are used as primers to amplify SSX2 DNA sequence using pET9a24aSSX2 as a template. By using these primers, the

Using PCR technique, the two nucleotides are used as primers to amplify SSX2 DNA sequence using pET9a24aSSX2 as a template. By using these primers, the restriction sites and six His-tag genes are appended onto SSX2 genes giving rise to Nde1-NHSSX2-Not1 DNA fragments. After PCR, Nde1-NHSSX2-Not1 is presented in the form of double-stranded DNA fragments. Then, Nde1-NHSSX2-Not1 fragments are cloned into a cloning vector, pCR2.1 plasmid, to create plasmid pCR2.1-Nde1-NHSSX2-Not1. What is the purposed of this step? Explain why the researchers insert the gene in the cloning vector (pCR2.1) not in the expression vector (pET9a24a).

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!

Sunrise Healthcare is a physician specialty practice located in Tennessee. Dr. DeMarco, the lead physician manager of the practice, recently learned that two former employees of Sunrise Healthcare...

Question: Genomic sequence of gene of interest:...

Question 5 [20] a) Given the messages of user A and B are "101" and 011 respectively, i.e., User A's message: "101" and User B's message: "011. The two messages will be transmitted by using CDMA...

(6) Kelvin is solving a demand-capacity problem using transportation modeling. He obtained the result as in Table 6, however the solution is not optimum. [Kelvin sedang menyelesaikan masalah...

According to a CIMA research report from 2006, many manufacturing companies continue to use traditional management accounting techniques such as absorption costing. The research explored 41 UK...

Hello, I would like a presentation to be made for 2 sections from the attached article! DOES THE CHOICE OF EXCHANGE RATE REGIME AFFECT THE ECONOMIC GROWTH OF DEVELOPING COUNTRIES? Glauco De Vita...

5. Label three 0.2 mL sterile PCR tubes [3 per student / 9 per group] of respective color with corresponding labels to the MM/PCR reactions you will prepare. Follow the table above. Keep tubes closed...

Which type of PCR do some laboratories use to detect simultaneously two or more different targets from one PCR tube? This technique utilizes two different primer sets and is often used to detect an...

You have collected a sample from a fresh water stream and want to check the presence of a specific virulence gene that belong to a pathogenic bacteria with the aid of the polymerase chain reaction...

Each answer per question must be 200 words or more, you must cite/reference your sources Part A: What is a Trace Metal Detection Test? Explain why one is conducted. Part B: What is a PCR analysis?...

It may be that in the Amazon rain forest there is a species of arrow frog that has three skin color variants. Sampling in a one kilometer by ten kilometer strip of forest gave the following counts: 2...

Take any 5 LARGE numbers from the article and write them in SCIENTIFIC NOTATION. After reading the section "If Time Were Money" discuss what this means to you. How are the billionaires getting their...

A circular ring of radius 200 mm is pinned at A and is supported by rod BC, which is fitted with a collar at C that can be moved along the ring. For the position when = 35, determine (a) The force...

The current 3 - month spot interest rate is equal to 4 . 0 0 % . The current 6 - month spot interest rate is equal to 4 . 2 5 % . What is the non - arbitrage the 3 X 6 FRA interest rate?

There are three parts to this question. a) Suppose a process has (effective) user-id (UID) 1000 and (effective) group-id (GID) 2000. Further suppose that an ordinary file example is owned by user...

LO23.2 Discuss the extent and sources of income inequality.

14. last word What groups benefit and what groups lose from the U.S. sugar subsidy program?

2. Suppose that both wheat and corn have an income elasticity of 0.1. LO22.1 a. If the average income in the economy increases by 2 percent each year, by what percentage does the quantity demanded of...