Using the attached sequences in FASTA format that are ordered indistinctly to the number that species have,

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Question:

Using the attached sequences in “FASTA” format that are ordered indistinctly to the number that species have,

a) Choose one of the sequences, perform a “Blast” with the NCBI resources and with the Expasy Blast Resources. Establish the differences between these resources.

b) Determine to which genera the 7 attached sequences of the protein belong myoglobin. // Use the “alignment” resources found at NCBI.

a) Perform an alignment of all the given sequences (Multiple Alignment), notice the similarities / differences and discuss about it.

b) Present the alignment obtained.

c) Calculate the molecular weight and isoelectric point of the given proteins.

Genders:
1. Gorilla gorilla beringei, => Sequence name ___________
2. Callithrix jacchus, => Sequence name ___________
3. Phoca sibirica, => Name of the sequence ___________
4. Bison bison (American bison), => Sequence name ___________
5. Didelphis marsupialis virginiana, => Sequence name ___________
6. Balaenoptera physalus, => Sequence name ___________
7. Homo sapiens, => Name of the sequence ___________

>Protein_1

MVLTDAEWHLVLNIWAKVEADVAGHGQDILISLFKGHPETLEKFDKFKHLKTEAEMKASEDLKKHGNTVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSRHPADFGADAQAAMNKALELFRKDIAAKYKELGFQG

>Protein_2

MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLERFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQRAMNKALELFRKDMASNYKELGFQG

>Protein_3

MGLSDGEWQLVLNVWGKVEADIPSHGQEVLISLFKGHPETLEKFDKFKHLKSEDEMKASEELKKHGVTVLTALGGILKKKGHHEAELKPLAQSHATKHKIPVKYLEFISDAIVHVLQKKHPGDFGADAQGAMKKALELFRNDMAAKYKELGFQG

>Protein_4

MGLSDGEWHLVLNVWGKWETDLAGHGQEVLIRLFKSHPETLEKFDKFKHLKSEDDMRRSFDLRKHGNTVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISEAIIHVLHSKHPAEFGADAQAAMKKALELFRNDIAAKIKELGFHG

>Protein_5

MGLSDGEWQLVLNAWGKVEADVAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASEDLKKHGNTVLTALGGILKKKGHHEAEVKHLAESHANKHKIPVKYLEFISDAIIHVLHAKHPSDFGADAQAAMSKALELFRNDMAAQYKVLGFHG

>Protein_6

MGLSDGEWQLVLNAWGKVEADVAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASEDLKKHGNTVLTALGGILKKKGHHEAEVKHLAESHANKHKIPVKYLEFISDAIIHVLHAKHPSDFGADAQAAMSKALELFRNDMAAQYKVLGFHG

>Protein_7

MGLSDGEWQLVLNAWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGNILKKKGNHEAELKPLAQSHATKHKISVQFLEFISEAIIQVIQSKHPGDFGGDAQAAMGKALELFRNDMAAKYKELGFQG