Question: using the data table provided, please help determine the substrate concentration (c1v1=c2v2). ONPG was our substrate, the original concentration in our manual is listed as
using the data table provided, please help determine the substrate concentration (c1v1=c2v2). ONPG was our substrate, the original concentration in our manual is listed as 50mL per group stock solution of 200M ONPG. Volume added is in column 3 of table and final volume in column 5. Also, how would I find the moles of product formed? Thank you.

Subsection 2B: Enzyme Assay 1) Gather and label tubes 1-10: 2) Set up the following reaction tubes, omitting the lactase. Enzyme Kinetics Lab Last Modified Jan 23, 2023
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