Using the fed-batch data in the excel spreadsheet provided, calculate (a) cell-specific growth rate and doubling time, (b) cell-specific glucose consumption and lactate production rates, and (c) the cell-specific IgG production rates. Calculate these parameters using differential and integral methods. For (a) and (b) focus only on the exponential phase of the growth while (c) perform the analysis for the whole culture period.

Days in Culture	Viable cell density	Viability	Nova pH	Nova Glucose (Gluc) g/L	Nova Lactate (Lac) g/L	Titer (mg/L)
0.0	1.87E+05	90.30	7.201	6.1	0	0
1.4	3.86E+05	95.20	7.042	6	0.05	18.05
2.3	6.40E+05	93.90	7.074	5.8	0.1	29.55
2.7	8.00E+05	93.80	7.064	5.5	0.3	36.65
3.3	1.15E+06	93.60	6.916	5.2	0.5	58.5
3.9	1.73E+06	93.60	6.639	4.75	0.8	65
4.5	2.40E+06	88.80	6.688	4.1	1.1	70.5
5.0	3.76E+06	92.40	6.698	3.1	1.8	135.5
5.7	4.94E+06	93.20	6.683	2	2.8	189.5
6.2	6.70E+06	92.10	6.666	0.5	3.89	250.5
6.9	8.09E+06	91.60	6.692	0	4.1	368.5
7.4	8.86E+06	88.70	6.743	0	4.05	495
8.1	1.02E+07	87.70	6.73	0	4	641.5
8.6	1.17E+07	87.00	6.75	0	3.8	767.6
9.5	1.23E+07	84.60	6.885	0	3.76	962.5
9.9	1.24E+07	81.80	6.81	0	3.7	1148
10.6	1.35E+07	79.10	6.851	0	3.44	1366
11.1	1.31E+07	76.10	6.851	0	3.4	1575
11.8	1.27E+07	72.70	6.964	0	3.2	1756
12.3	1.17E+07	63.70	6.919	0	3.4	1962
12.9	9.84E+06	59.20	6.961	0	3.2	2112
13.4	8.87E+06	47.90	6.985	0	3.12	2215
14.1	7.15E+06	40.80	7.044	0	3	2328

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ANSWER a Cellspecific growth rate and doubling time Differential method The cellspecific growth rate can be calculated using the following equation lnN2N1t2t1 where N1 is the viable cell density at ti... View the full answer