Question: Using the fed-batch data in the excel spreadsheet provided, calculate (a) cell-specific growth rate and doubling time, (b) cell-specific glucose consumption and lactate production rates,

Using the fed-batch data in the excel spreadsheet provided, calculate (a) cell-specific growth rate and doubling time, (b) cell-specific glucose consumption and lactate production rates, and (c) the cell-specific IgG production rates. Calculate these parameters using differential and integral methods. For (a) and (b) focus only on the exponential phase of the growth while (c) perform the analysis for the whole culture period.

Days in CultureViable cell densityViabilityNova pHNova Glucose (Gluc) g/LNova Lactate (Lac) g/LTiter (mg/L)
0.01.87E+0590.307.2016.100
1.43.86E+0595.207.04260.0518.05
2.36.40E+0593.907.0745.80.129.55
2.78.00E+0593.807.0645.50.336.65
3.31.15E+0693.606.9165.20.558.5
3.91.73E+0693.606.6394.750.865
4.52.40E+0688.806.6884.11.170.5
5.03.76E+0692.406.6983.11.8135.5
5.74.94E+0693.206.68322.8189.5
6.26.70E+0692.106.6660.53.89250.5
6.98.09E+0691.606.69204.1368.5
7.48.86E+0688.706.74304.05495
8.11.02E+0787.706.7304641.5
8.61.17E+0787.006.7503.8767.6
9.51.23E+0784.606.88503.76962.5
9.91.24E+0781.806.8103.71148
10.61.35E+0779.106.85103.441366
11.11.31E+0776.106.85103.41575
11.81.27E+0772.706.96403.21756
12.31.17E+0763.706.91903.41962
12.99.84E+0659.206.96103.22112
13.48.87E+0647.906.98503.122215
14.17.15E+0640.807.044032328











Step by Step Solution

3.34 Rating (169 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer
Step: 1 Unlock

ANSWER a Cellspecific growth rate and doubling time Differential method The cellspecific growth rate can be calculated using the following equation lnN2N1t2t1 where N1 is the viable cell density at ti... View full answer

blur-text-image
Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock
Step: 3 Unlock

Students Have Also Explored These Related Accounting Questions!