v$)$ State whether you would see Mtb$7$ mRNA being produced or not produced in the conditions below, if cells were treated with LigA. $(2$ points each$)$

a$)$ Addition of a drug that inhibits the ability of Ras to exchange GDP for GTP$.$

b$)$ If PKG was constitutively active. $$

c$)$ If normal Ash$3$ was replaced with Ash$3$ that had Y$25$ changed to alanine. $$

d$)$ Replacement of normal Ras with a constitutively active Ras. $$

vi$)$ You have cells that have constitutively active Ras and add both LigA and a drug that prevents Ash$3$ from binding to DNA. How do you predict this would affect Mtb$?$ expression? Explain. $(3$ points$)$