What is the sample size in our sunstalk experiment? Consider the total number of sunstalks, but also the number of sunstalks of each genotype that will be divided between the treatments. How many independent observations will be made. A. The sample size is 432 sunstalks because we have 432 sunstalks in the experiment. B. The sample size is 206 sunstalks because we have 206 sunstalks in each environmental treatment. C. The sample size is 144 sunstalks because we have 144 sunstalks of each genotype in the experiment. D. The sample size is 72 sunstalks because we have 72 sunstalks of each genotype in each environmental treatment. E. The sample size is 1 sunstalk because we have 1 sunstalk of each genotype in each environmental treatment in each experimental block.

step s. The importance of sample size in an experiment Replication is a process in which multiple subjects are given the same treatment independently. It is important to have replicates, because different individuals may have slightly different responses to the same treatment. A replicate is one experimental unit in one treatment group. The number of replicates (n) is the number of experimental units in a treatment group. In our case we are interested in both the genotype AND the effect of the treatment. In the example below there are three metal tolerant replicates in treatment group 1. Metal tolerant (T) Metal Treatment group 1 Treatment group 2 intolerant (1) Click to add speaker notes