What two purification steps below will give you the best result for separation of a functional protein of 80,000 Da with a pl =7 in a mixture that contains other proteins with molecular weights of 20,00040,000 and a wide range of pl values. (A) Ammonium sulfate precipitation / centrifugation (B) Gel Filtration chromatography (C) Ion exchange chromatography (D) Affinity chromatography Calculate G for the reaction A+BC+D at 25C when the reagent and product concentrations are [A]=5M,[B]=40M,[C]=50M,[D]=0.001mM. (A) 813J/mol (B) 3.43kJ/mol (C) 3767J/mol (D) 9694J/mol