When primers (oligonucleotides) arrive at the lab from thecompany, they are typically shipped dry, and we need
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When primers (oligonucleotides) arrive at the lab from thecompany, they are typically shipped dry, and we need to resuspendthem in an aqueous solution before use. We usually add a volume ofTE buffer or water for resuspension to obtain a final concentrationof 100 μM (stock solution). As you can see in thisprotocol, the working solution of each primeris 2 μM (see the table below).
Indicate your calculations to prepare a workingsolution of the reverse primer at a concentration of 2 μM from thestock solution, consider the volume of primer needed per PCRreaction (per student), and that there are 24 students in your labsection
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