When you purchase T 4 DNA Ligase, a 10X buffer (400mM Tris, pH 7.5; 200mM MgCl 2
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When you purchase T4 DNA Ligase, a 10X buffer (400mM Tris, pH 7.5; 200mM MgCl2, 100mM dithiothritol, 70mM ATP, 450ug/ml bovine serum albumin) is supplied with it. What are the final concentrations of MgCl2 and bovine serum albumin when you use the ligase enzyme to perform a ligation reaction?
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ISBN: 978-0471726340
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Authors: Karen L. Hooks
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