Question: When you purchase T 4 DNA Ligase, a 10X buffer (400mM Tris, pH 7.5; 200mM MgCl 2 , 100mM dithiothritol, 70mM ATP, 450ug/ml bovine serum

When you purchase T₄ DNA Ligase, a 10X buffer (400mM Tris, pH 7.5; 200mM MgCl₂, 100mM dithiothritol, 70mM ATP, 450ug/ml bovine serum albumin) is supplied with it. What are the final concentrations of MgCl₂ and bovine serum albumin when you use the ligase enzyme to perform a ligation reaction?

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