which method would be the least reliable and why?

- Dye binding method (bradford method) of protein determination

-bicinchoninic acid (bca) method for protein determination

- lowry method of protein determination

- biuret method of protein determination

- absorbance at 280 and 260 nm

C. You are trying to study a protein called Ribonuclease A. After purifying it from from bovine (cow) pancreatic cells you want to determine the concentration of your sample. Ribonuclease A has zero Tryptophan residues and few Tyrosines and Phenylalanines residues. Of the options in section 4.4 of your book, which method would be the least reliable for determining Ribonuclease concentration? Include a rationale for your