Question: Who Did That? This person is attributed with developing hand washing. ________________ This person is attributed with founding nursing and bringing aseptic techniques to nursing.
Who Did That?
This person is attributed with developing hand washing. ________________
This person is attributed with founding nursing and bringing aseptic techniques to nursing. ________________
This person used a microscope to discover the first cell. _______________
This person found the first bacteria under the lens. _______________
This scientist developed 4 postulates for identifying causative pathogens. ________________
This scientist performed an S-shaped flask experiment to support biogenesis. ________________
This French microbiologist worked on chicken cholera, anthrax, swine erysipelas, and rabies_______________
Surgeon who attributed with developing instrument sterilization and wound sanitation. ______________
The father of taxonomy. ___________________
Host-Microbial interaction
Most microbes are helpful or neutral to humans, but _p-____________ bacteria cause disease. All host microbial interactions are _____________. The normal microbiota of the colon is an example of a _____________ relationship. The temporary bacteria on the surface of the skin that provide no benefit but no real cost to us, would be considered a _______________ relationship to humans. Pathogenic bacteria have a _____________ relationship to humans.
What's That Stain Again?
This staining technique identifies whether a bacterium has a thick or thin peptidoglycan layer. _____________
If a bacterium is positive for this technique, the bacteria shows up violet. ___________________
This stain is used for bacteria with high content of mycolic acid in their cell walls. ___________________
These stains are important in determining what antimicrobial or treatment is needed. __________________
This type of stain identifies an inner structure in an especially hardy type of bacteria. __________________
This type of stain is helpful only to identify a bacteria's basic morphology. ___________________
If a bacterium has a sticky carbohydrate-based structure that prevents most stains from setting up, a _______________ may be in order.
The staining of an external organelle that is used for motility. __________________
Pro or Eu or both?
Only unicellular ________________ Contains peptidoglycan ________________
Contains DNA ________________ Can possess a cell wall ________________
Contains RNA ________________ Can be multicellular __________________
Use Binary Fission ______________ Ribosomes_________________
Contains a nucleus ________________ Yeast _______________
Some species have endospores _______________ Protozoans ________________
Contain mitochondria________________ Contains a Cytoskeleton__________________
Name That Organelle.
This organelle is found in Eu- and Prokaryotes, important for protein synthesis _________________
DNA is stored here in eukaryotes. ______________
Considered the USPS of the cell. _________________
Important for motility in prokaryotes. ________________ More numerous than pili, these are external organelles of prokaryotic cells for adhesion _______________
Main component of the slime layer and also capsules; helps with adhesion and protection _________________ Important for processing some toxins and lipid synthesis ______________________ Mycoplasma have a sterol enriched version of this structure ____________________ These organelles are used during times of plenty to store materials in prokaryotes. _______________ Similar to mitochondria, plants (always) and protists (sometimes) contain this organelle. __________________
Which Eukaryote?Plants, Animals, Protist, Fungi
This category contains amoeboids. ______________
Infections from this branch are called mycoses ____________
Tinea, Yeast, and mostly opportunistic infections a characteristic of this eukaryote. _____________
These special microscopic eukaryotes can contain flagella or cilia. _________________
Helminths/parasitic worms __________________
Included in microbiology due to certain stages of the life cycle being microscopic (aka protozoans)._________
Only a vehicle for infectious agents. _________________
Most of this grow as a collection of tubular structures called hyphae ______________
Important Definitions
Unicellular and lack a membrane-bound nucleus _________________
Core component of bacterial cell walls ________________________
Waxy lipid found on bacteria starting with "myco-" _________________
Type of cell form that endospores enter when conditions become favorable again _________________
"Cell Eating" _________________ "Cell Drinking" _______________
Removing things from the cells via vesicles ________________ Plating technique for isolating a specific species of microbes for study. ________________ Applying heat or chemicals to adhere a smear (sample) to the slide. __________________
The goal of streak plate technique ______________________
General term for stains that highlight differences in bacterial cell walls in order to discriminate between classes of cells ________________
Purpose of applying oil is to decrease this term that means bending of light. ____________________
This type of Electron Microscope does not have the best magnification but gives 3D _______________
Emission of energy as light, usually UV. _____________________
Sticky communities made up of a single or diverse microbial species. ______________
Free-floating bacteria _________________
Genetic variants of the same species (mostly bacteria and viruses) ___________
Agents that cause disease in humans ____________________
Another word for Healthcare-acquired infections (HAIs) ________________________
Causative or ____________ agent. Pathogens that only cause disease in a weakened host. ____________________ 1850-1920 The _________________ Age of Microbiology.
Cellular and non-cellular entities often not seen with the naked eye _______________
Even though the history books ____________ a discovery to a scientist, it does not mean they are first.
Morphologies
Arranged in pairs________________ Clusters of bacilli__________________
Arranged in a string______________ Clusters of cocci__________________
Look like beads __________ Comma ____________ Rods _____________ Star-shaped ___________ Spirals ________________ Different Forms _______________
LAB COMPANION (Leave Pages 1 & 2 in other classroom):
Week 1 Activity Schedule Checklist:
- Look a 5 stock slides, 1 slide on 100x.
- Gram Stain 1 slide
- Swab a surface and perform streak plate technique
Fill-in Microscope Directions:
- Broke slides are sharp, this means slides can _______ skin.
- Any injury should be reported to ______________________.
- When using the microscope, ________ adjustment can be tricky. Asking the professor for help is encouraged.
- The course focus knob is used to find "_____________".
- The __________ focus knob is used once you have color.
- Microscopy is a science and an _____. Do not get discouraged if you are having difficulty.
- If you wish to use the 100x objective lens, _____ must be used.
- While adjusting focus make sure not to ________________ the slide. 2 broken slides in one day due to ramming the lens into the slide will result in __________ of microscope privileges for the day
- Our microscopes have an ocular lens of ___ x magnification. If we are on the 40x objective lens, what is the total magnification? ___________
- Moving the objective lens ___________ is the _______ way to change objective lens. Multiple infractions will result in loss of microscope privileges for the day.
Staining KEY Directions (not comprehensive):
Following the comprehensive of directions step-by-step. Questions of "What do I do?" will not be answered. Questions about the current step you are on will gladly be answered!
Abbreviated directions (Not complete)
Step 1. Smear your ______. Then wait for it to dry.
Step 2. _____ your slide. Step 3. ______ stain your slide.
Step 4. Perform __________ to ID whether your bacteria is Gram Positive or Gram Negative. You should also look at the size, shape, and arrangement of your bacteria (aka ________________)
Step 5: Put stock slides in designated area. Place all created slides in the __________ container.
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