Question: Who Did That? This person is attributed with developing hand washing. ________________ This person is attributed with founding nursing and bringing aseptic techniques to nursing.

Who Did That?

This person is attributed with developing hand washing. ________________

This person is attributed with founding nursing and bringing aseptic techniques to nursing. ________________

This person used a microscope to discover the first cell. _______________

This person found the first bacteria under the lens. _______________

This scientist developed 4 postulates for identifying causative pathogens. ________________

This scientist performed an S-shaped flask experiment to support biogenesis. ________________

This French microbiologist worked on chicken cholera, anthrax, swine erysipelas, and rabies_______________

Surgeon who attributed with developing instrument sterilization and wound sanitation. ______________

The father of taxonomy. ___________________

Host-Microbial interaction

Most microbes are helpful or neutral to humans, but _p-____________ bacteria cause disease. All host microbial interactions are _____________. The normal microbiota of the colon is an example of a _____________ relationship. The temporary bacteria on the surface of the skin that provide no benefit but no real cost to us, would be considered a _______________ relationship to humans. Pathogenic bacteria have a _____________ relationship to humans.

What's That Stain Again?

This staining technique identifies whether a bacterium has a thick or thin peptidoglycan layer. _____________

If a bacterium is positive for this technique, the bacteria shows up violet. ___________________

This stain is used for bacteria with high content of mycolic acid in their cell walls. ___________________

These stains are important in determining what antimicrobial or treatment is needed. __________________

This type of stain identifies an inner structure in an especially hardy type of bacteria. __________________

This type of stain is helpful only to identify a bacteria's basic morphology. ___________________

If a bacterium has a sticky carbohydrate-based structure that prevents most stains from setting up, a _______________ may be in order.

The staining of an external organelle that is used for motility. __________________

Pro or Eu or both?

Only unicellular ________________ Contains peptidoglycan ________________

Contains DNA ________________ Can possess a cell wall ________________

Contains RNA ________________ Can be multicellular __________________

Use Binary Fission ______________ Ribosomes_________________

Contains a nucleus ________________ Yeast _______________

Some species have endospores _______________ Protozoans ________________

Contain mitochondria________________ Contains a Cytoskeleton__________________

Name That Organelle.

This organelle is found in Eu- and Prokaryotes, important for protein synthesis _________________

DNA is stored here in eukaryotes. ______________

Considered the USPS of the cell. _________________

Important for motility in prokaryotes. ________________ More numerous than pili, these are external organelles of prokaryotic cells for adhesion _______________

Main component of the slime layer and also capsules; helps with adhesion and protection _________________ Important for processing some toxins and lipid synthesis ______________________ Mycoplasma have a sterol enriched version of this structure ____________________ These organelles are used during times of plenty to store materials in prokaryotes. _______________ Similar to mitochondria, plants (always) and protists (sometimes) contain this organelle. __________________

Which Eukaryote?Plants, Animals, Protist, Fungi

This category contains amoeboids. ______________

Infections from this branch are called mycoses ____________

Tinea, Yeast, and mostly opportunistic infections a characteristic of this eukaryote. _____________

These special microscopic eukaryotes can contain flagella or cilia. _________________

Helminths/parasitic worms __________________

Included in microbiology due to certain stages of the life cycle being microscopic (aka protozoans)._________

Only a vehicle for infectious agents. _________________

Most of this grow as a collection of tubular structures called hyphae ______________

Important Definitions

Unicellular and lack a membrane-bound nucleus _________________

Core component of bacterial cell walls ________________________

Waxy lipid found on bacteria starting with "myco-" _________________

Type of cell form that endospores enter when conditions become favorable again _________________

"Cell Eating" _________________ "Cell Drinking" _______________

Removing things from the cells via vesicles ________________ Plating technique for isolating a specific species of microbes for study. ________________ Applying heat or chemicals to adhere a smear (sample) to the slide. __________________

The goal of streak plate technique ______________________

General term for stains that highlight differences in bacterial cell walls in order to discriminate between classes of cells ________________

Purpose of applying oil is to decrease this term that means bending of light. ____________________

This type of Electron Microscope does not have the best magnification but gives 3D _______________

Emission of energy as light, usually UV. _____________________

Sticky communities made up of a single or diverse microbial species. ______________

Free-floating bacteria _________________

Genetic variants of the same species (mostly bacteria and viruses) ___________

Agents that cause disease in humans ____________________

Another word for Healthcare-acquired infections (HAIs) ________________________

Causative or ____________ agent. Pathogens that only cause disease in a weakened host. ____________________ 1850-1920 The _________________ Age of Microbiology.

Cellular and non-cellular entities often not seen with the naked eye _______________

Even though the history books ____________ a discovery to a scientist, it does not mean they are first.

Morphologies

Arranged in pairs________________ Clusters of bacilli__________________

Arranged in a string______________ Clusters of cocci__________________

Look like beads __________ Comma ____________ Rods _____________ Star-shaped ___________ Spirals ________________ Different Forms _______________

LAB COMPANION (Leave Pages 1 & 2 in other classroom):

Week 1 Activity Schedule Checklist:

  • Look a 5 stock slides, 1 slide on 100x.

  • Gram Stain 1 slide

  • Swab a surface and perform streak plate technique

Fill-in Microscope Directions:

  1. Broke slides are sharp, this means slides can _______ skin.
  2. Any injury should be reported to ______________________.
  3. When using the microscope, ________ adjustment can be tricky. Asking the professor for help is encouraged.
  4. The course focus knob is used to find "_____________".
  5. The __________ focus knob is used once you have color.
  6. Microscopy is a science and an _____. Do not get discouraged if you are having difficulty.
  7. If you wish to use the 100x objective lens, _____ must be used.
  8. While adjusting focus make sure not to ________________ the slide. 2 broken slides in one day due to ramming the lens into the slide will result in __________ of microscope privileges for the day
  9. Our microscopes have an ocular lens of ___ x magnification. If we are on the 40x objective lens, what is the total magnification? ___________
  10. Moving the objective lens ___________ is the _______ way to change objective lens. Multiple infractions will result in loss of microscope privileges for the day.

Staining KEY Directions (not comprehensive):

Following the comprehensive of directions step-by-step. Questions of "What do I do?" will not be answered. Questions about the current step you are on will gladly be answered!

Abbreviated directions (Not complete)

Step 1. Smear your ______. Then wait for it to dry.

Step 2. _____ your slide. Step 3. ______ stain your slide.

Step 4. Perform __________ to ID whether your bacteria is Gram Positive or Gram Negative. You should also look at the size, shape, and arrangement of your bacteria (aka ________________)

Step 5: Put stock slides in designated area. Place all created slides in the __________ container.

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