Question: Why are cells lysed during a DNA extraction? What is InstaGeneTM Matrix? Why did you use it during your DNA extraction? PCR is a powerful

Why are cells lysed during a DNA extraction?

What is InstaGeneTM Matrix? Why did you use it during your DNA extraction?

PCR is a powerful procedure because it can make many, many copies of a specific DNA sequence within a few hours. True or False

What happened in each of the three steps of the PCR reaction?

What enzyme did you use in your PCR reactions? Why is this enzyme remarkable?

What short DNA molecules direct DNA polymerase to the target DNA for amplification?

What monomers are included in the PCR master mix?

What machine was invented to automate PCR?

The small repetitive DNA element/sequence that you amplified by PCR is the ___ sequence.

How many cycles occurred in your PCR reaction?

Explain the purpose of the three control tubes in your PCR reaction.

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