Question: Why do researchers perform a partial digest, rather than a complete digest, of genomic DNA when making a genomic library? this creates overlapping fragments of
Why do researchers perform a partial digest, rather than a complete digest, of genomic DNA when making a genomic library?
this creates overlapping fragments of different sizes which is necessary for screening the library by chromosome walking
this creates sticky ends which are necessary for ligation to the plasmid
because it would take a very long time to perform a complete digest
this is necessary so that colonies containing recombinant DNA can be identified using bluewhite selection
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