Question: Why do we need to engineer an orthogonal tRNA/synthetase pair for the amber suppression method? Select all that apply; no partial credit. Because otherwise the

Why do we need to engineer an orthogonal tRNA/synthetase pair for the amber suppression method? Select all that apply; no partial credit. Because otherwise the natural synthetases will charge the new IRNA with canonical amino acids Because otherwise the new tRNA will be degraded by the cell Because otherwise the new synthetase will charge endogenous IRNAs with the ncAA Because otherwise the new synthetase will not be functional in cells

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