WORK SHEET: EFFECT OF FLOW RATE ON COLUMN PERFORMANCE

IInclude an example of all calculations and send copy of your excel worksheet to your instructor

a$.$ the true flow rates for the five "Expected Flow retes"

b$.$ Complete the following table. Can use Excel, but must show equations applied.

c$.$ Complete the following table $($e$.$g$.N=$ the number of theoretical plates$)$ for each compound,

at each flow rate, using $w$ and $w_{is}$

d$.$ Complete the table $($e$.$g$.H=$ height equivalent per theoretical plate$)$ for each retained species,

using $w$ and $w$ w $\frac{1}{2}$

e$.$ Review the "peak asym metry valuesiajur, in the table below, are they within the acceptance

parameters? Explain

Van Deaptarplots:

The most retained compound in this chrom atographic separationsis:

$(1$ pt$)$

b$.$ Draw a van Degmter plot of $H$ vs$.$ real flow rate for the mostretained compound ONLY.

Identify the optimum flow rate using the graph? $(2$ pts$)$e$.$ Review the "peak asymmetry values'given in the table below, are they within the acceptance

parameters? Exnlain

Van Deamterplots:

a$.$ The most retained compound in this chrom atographic separationis:

b$.$ Draw a van Deemter plot of $H$ vs$.$ real flow rate for the mostretained compound ONLY.

Identify the optimum flow rate using the graph? $(2$ pts$)$

c$.$ Draw a van Deenterplot of $H_{\frac{1}{2}}vs.$ real flow rate for the mostretained compound ONLY.

Identify the optimum flow rate using the graph? $(2$ pts$)$

d$.$ Accordina to your van Deeontecplots $(3b$&$3c),$ indicate which is the optimum flow rate for the

separation?