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Collect POST- DRE urine in a Urine Protocol sterile cup and place on ice Transfer the first 30mL into a 50ml tube (for Invert 3-5 RNALater) and the remainder into another 50mL Remainder times tube (for PBS). Add 15.0mL to Procure F. 10 ml a 50mL tubes Centrifuge for 10 min at 1000g at 4 C conical tube Aliquot 4mL into 2x2mL Centrifuge Pellet for 10 min Supernatant Add 15.0mL of labeled tubes for Tissue at 4000 rpm mUTM to the Core at 4 C Add 5mL of ice cold 1X PBS and 50mL conical Aliquot re-suspend with a sterile pipette tube and mix 10mL into Transfer the 2x5mL sediment to tubes and Transfer to a 15mL Aliquot .5.0 mL 1.7 ml tube label for conical tube into 6x5mL Metabolon Store at -80 C labeled tubes Centrifuge for 10 min at 1000g at 4 C Store at -80 C Microfuge for 5 Store at -80 C min at 12000rpm at Discard 4 C Discard supernatant remainder of Supernatant Decant, Add 1ml of ice cold 1X PBS and re-suspend with a supernatant not sterile pipette and add to a 1.7mL eppendorf tube. to disturb pellet. Split the PBS only sample into 2x0.5mL tubes Store at -80 C Microfuge for 10 min at 700g at 4 C Decant RNALater sample Add 0.250mL of RNAlater and mix (vortex) updated 2/6/2019 Store at 4 C for 24+ hours Split into 2x0.125mL aliquots and store at - 80 C