Write the formulas and calculations required to perform each step.

1. Prepare 1 L of a 0.05 M H2SO4 solution (2.5 mL of concentrated H2SO4 in 1 L of nanopure water).

2. Prepare a standard solution of 100 ppm quinine. The form of

quinine that is most used is the standard quinine sulfate (mono or dihydrate) and it is necessary to make the calculations to consider the weight that does not come from quinine. After doing the calculation, (you will weigh more of 25 mg), dissolve it in H2SO4 0.05 M and transfer quantitatively to a 250 mL volumetric flask. Complete up to the mark with H2SO4 0.05 M.

3. Prepare 4 standard quinine solutions from the standard solution with final concentrations of 0.5

ppm, 1.00 ppm, 1.50 ppm and 2.0 ppm. Use 100 mL graduated flasks and 0.05 M H2SO4 as solvent.

Read the fluorescence intensity of each of these solutions.

4. Get a tonic water sample and dilute 1:10 with H2SO4 0.05 M. in 100 mL. Then prepare

another dilution from that, adding 15 mL in 100 mL and completing the brand with H2SO4 0.05 M. Read

The fluorescence intensity of this solution. Make the necessary dilutions so that the reading of

Fluorescence of your sample is within the linear range of the method.