Question: You are cloning using the pUC 1 9 vector. After transforming E . coli cells and plating them in the presence of ampicillin, you realize
You are cloning using the pUC vector. After transforming coli cells and plating them in the presence of ampicillin, you realize that you forgot to add XGal to your plate. What would you expect to observe and is it possible to visually identify bacteria that have taken up the plasmid with the DNA insert?
There would be blue colonies and white colonies on the plate. The bacteria that have taken up the plasmid with DNA insert can be identified by their blue color.
All colonios on the plate vould be vethite. You would not be able to identify which bactientia torote up the plasmid with DNA insert.
All colonies on she pliate woridibe white, if the colony is growing in the presence of ampicilins in bas baken up the plasmid containing the DNA insert.
identity which bacteria tock tre the glasmid wich DisA insert.
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