Question: You are studying a protein complex that has 4 different protein chains A(15 kDa), B(13 kDa), C(12 kDa), and D(10 kDa) a. Using size exclusion

You are studying a protein complex that has 4 different protein chains

A(15 kDa), B(13 kDa), C(12 kDa), and D(10 kDa)

a. Using size exclusion chromatography, you see this complex is approximately 100 kDa. How is this possible?

b. You increase the salt concentration and run size exclusion chromatography again, You collect two peaks, Fraction 1 at 50kDa and Fraction 2 at 25 kDa. You run SDS-PAGE of these fractions and see that Fraction 1 contains A and D while Fraction 2 contains chains B and C. Explain this data.

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!

The unadjusted trial balance of Recessive Interiors at January 31, 2019, the end of the year, follows: The data needed to determine year-end adjustments are as follows: a. Supplies on hand at January...

List four basic financial statement analysis procedures, describe how you would calculate each procedure and discuss why you would use each procedure. In your initial response, please do not use...

There are two problems due this week (each worth 35 points) as follows. Problem 1.6 (page 20) In comprehensive paragraphs, answerrequirements a to e. You will have 5 paragraphs total of four to five...

07-043 March 13, 2008 Eli Lilly: Recreating Drug Discovery for the 21st Century Rebecca Henderson and Cate Reavis The rise of personalized medicine is one of the most important developments in health...

The instructions are attached in the Division Performance Evaluation Division Performance Evaluation Project Using Excel In this assignment you will analyze the performance of PepsiCo or Marriott's...

tudy of an innovative method based on complementarity between ARIZ, lean management and discrete event simulation for solving warehousing problems Fatima Zahra Ben Moussa a, , Roland De Guiob ,...

Would you be able to put together another monthly cash flow analysis, this time for year 1? INCOME STATEMENT Year 1 269,115.00 48,930.00 20,388.00 338,433.00 $ $ $ $ COGS $ 113,940.00 $ 125,940.00 $...

KINGS OWN INSTITUTE* Success in Higher Education ICT106 DATA COMMUNICATIONS AND NETWORKS T223 Page 1 of 18 AUSTRALIAN INSTITUTE OF BUSINESS AND MANAGEMENT PTY LTD ABN: 72 132 629 979 CRICOS 03171A...

please help with below question as per the attached article on Using Supply Chain Analysis to Examine the Costs of NonTariff Measures (NTMs) : Michael J Ferrantino, WTO 1. Critically examine the...

# 45 (use Stapplet) 51,57, 59(Stapplet), 61, 69(Stapplet), 75, 80-85 52 CHAPTER 1 DATA ANALYSIS these cereal brands Between what two values 77. Birth does it her student (ed) About what percent of...

By MARK@ (Ruska, Markevichus, Naniev, Grachev) Emerging russian ice-cream market is good area for different producers as it has great potential 1. Currently Russian ice-cream market is the 4th...

1. What financial risks is Vicky assuming? 2. What are the risks that are specific to renting motor scooters? 3. What are the risks specific to motor scooters, as physical assets? 4. What risks will...

Gotham University offers only high-tech graduate-level programs. Gotham has two principal operating departments, Engineering and Computer Sciences, and two support departments, Facility and...

Harvesting owners can be paid in cash or in the stock of the acquiring firm, with stock generally being preferred over cash. True False

which requirement suggests configuring amazon route 5 3 with latency routing

=+ How much do the major football or basketball programs contributedirectly or indirectly to other parts of a typical university?

=+What is the local economic

=+company feels overworked; few people can imagine adding another