You are working in the R&D department of a pharmaceutical company and have developed a new...

 

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You are working in the R&D department of a pharmaceutical company and have developed a new small drug molecule for potential use in patients with Parkinson's disease. The compound, when dissolved in methanol, has a chromophore with two absorption maxima, the first at = 205 nm with a specific absorbance of A (1%, 1cm) = 1,800 and the second at A = 264 nm with a specific absorbance of A( 1%, 1 cm) = 392. You are asked to develop an assay using UV-Vis spectroscopy which can be used to routinely quantify the compound. a) Briefly discuss which of the two absorbance maxima you would deem more suitable for setting up the assay. b) Why is it important to know the specific absorbance of a compound in the context of pharmaceutical analysis and development of a method to routinely quantify the compound? c) The compound has low solubility in acidic aqueous medium. However, it dissolves well in alkaline aqueous solution in which a bathochromic shift is observed in its UV spectrum. What information about the chemical structure of the compound can be deduced from its solubility and UV absorbance at different pH? d) If the Amax was not affected by the change in pH but only the solubility, what would that tell you about the chemical structure? e) While working with the new drug, you notice that it exhibits fluorescence. How would you set up a quantification method to be used routinely which is based on the compound's fluorescence? You are working in the R&D department of a pharmaceutical company and have developed a new small drug molecule for potential use in patients with Parkinson's disease. The compound, when dissolved in methanol, has a chromophore with two absorption maxima, the first at = 205 nm with a specific absorbance of A (1%, 1cm) = 1,800 and the second at A = 264 nm with a specific absorbance of A( 1%, 1 cm) = 392. You are asked to develop an assay using UV-Vis spectroscopy which can be used to routinely quantify the compound. a) Briefly discuss which of the two absorbance maxima you would deem more suitable for setting up the assay. b) Why is it important to know the specific absorbance of a compound in the context of pharmaceutical analysis and development of a method to routinely quantify the compound? c) The compound has low solubility in acidic aqueous medium. However, it dissolves well in alkaline aqueous solution in which a bathochromic shift is observed in its UV spectrum. What information about the chemical structure of the compound can be deduced from its solubility and UV absorbance at different pH? d) If the Amax was not affected by the change in pH but only the solubility, what would that tell you about the chemical structure? e) While working with the new drug, you notice that it exhibits fluorescence. How would you set up a quantification method to be used routinely which is based on the compound's fluorescence?