Stock Working Erythrocin B Tubes 1 2 3 4 5 Sunset yellow Tubes 1 2 3...

 

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Stock Working Erythrocin B Tubes 1 2 3 4 5 Sunset yellow Tubes 1 2 3 4 5 Dye concentrations Erythrosin B (mm) Sunset yellow (mm) 0.9911 ± 0.100 0.9992 ±0.200 Wavelenght (nm) MW (g/mol) 522 Dye volume and concentration for calibration curve erythrosin B Working stock/mL Distilled water/mL Erythrosin B/μM Absorbance 10 8 6 4 2 wavelenght (nm) MW (g/mol) 482 879.86 10 8 6 4 2 0 2 4 6 8 452.37 Dye volume and concentration for calibration curve Sunset Yellow Working stock/mL Distilled water/mL Erythrosin B/μM Absorbance 0.315 0.243 0.179 0.123 0.066 0 2 4 6 8 0.900 0.727 0.536 0.360 0.187 The questions are: 1. Find the concentration of the laboratory stock solutions and the concentration of your working solutions 2. For each diluted dye sample: working stock volume, distilled water volume, concentration and absorption. 3. Construct a plot of absorbance Vs concentration using excel and assess your plot via linear regression. concentration value. Use this plot to interpolate a concentration result of your unknown and associated error around the concentration value. Stock Working Erythrocin B Tubes 1 2 3 4 5 Sunset yellow Tubes 1 2 3 4 5 Dye concentrations Erythrosin B (mm) Sunset yellow (mm) 0.9911 ± 0.100 0.9992 ±0.200 Wavelenght (nm) MW (g/mol) 522 Dye volume and concentration for calibration curve erythrosin B Working stock/mL Distilled water/mL Erythrosin B/μM Absorbance 10 8 6 4 2 wavelenght (nm) MW (g/mol) 482 879.86 10 8 6 4 2 0 2 4 6 8 452.37 Dye volume and concentration for calibration curve Sunset Yellow Working stock/mL Distilled water/mL Erythrosin B/μM Absorbance 0.315 0.243 0.179 0.123 0.066 0 2 4 6 8 0.900 0.727 0.536 0.360 0.187 The questions are: 1. Find the concentration of the laboratory stock solutions and the concentration of your working solutions 2. For each diluted dye sample: working stock volume, distilled water volume, concentration and absorption. 3. Construct a plot of absorbance Vs concentration using excel and assess your plot via linear regression. concentration value. Use this plot to interpolate a concentration result of your unknown and associated error around the concentration value.

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