You got sick while you were visiting Granny (from the "old country. Unfortunately, she believed in the curative powers of raw garlic extracted in potato wine. You did notice that after drinking several glasses of this concoction your cold went away, but you became concerned that this 'cure' might be a mutagen!

(a) Describe how you can use the Ames test to determine if this molecule is a mutagen. Show negative and positive controls.

(b) Explain the different components of the Ames test and what they are used for

(c) Explain how it might be possible to have false positives and how you would test for them.

(d) What if Granny's concoction is broken down into metabolites in the body and these metabolites are the cancer causing agent, not the concoction. How do you test for this?