You have been given a DNA fragment obtained from the genome of Bacillus thuringiensis below: 3CACTAACTGTCGCCAGGTCTGATAGACATATAACTGTTGGCGTACATAAGAAGGATCAAAAAA5 Additional
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Question:
- You have been given a DNA fragment obtained from the genome of Bacillus thuringiensis below:
- 3’CACTAACTGTCGCCAGGTCTGATAGACATATAACTGTTGGCGTACATAAGAAGGATCAAAAAA5’
- Additional tools are provided below.
- a) Provide the complementary strand for the parent strand (above) that would be synthesized during replication of the linear DNA fragment
- b) List the enzymes involved in this DNA replication
- c)Use the parent strand as a template to synthesize mRNA strand. Describe how the mRNA strand is generated and regulated. Indicate the direction of synthesis.
- d)Synthesize a polypeptide from the mRNA.
TOOLS:
- Primer: GUGAUU
- Codon table
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