Your group is a part of a research team that just received agrant from the National Institutes of Health to study cancer. As afirst step, you decide to clone p53, a human gene that is mutant inmany types of cancer cells.

1. After cutting the plasmid with HindIIIand NotI, how many pieces of DNA will you have?

Enter your answer as a whole number.

Transcribed Image Text:

Your group is a part of a research team that just received a grant map of the p53 PCR product showing study cancer. As a first step, restriction enzyme cut sites that is mutant in many types of varivor vena. Below is a diagram of the DNA product of a PCR amplification of the region of the human genome containing p53. The white region represents the coding region of the gene. The letters above the DNA represent the locations of specific restriction enzyme cut sites. The numbers below each restriction site represent the number of kilobases (kb) from the first restriction site (at 0 kb). Each of the restriction enzymes creates unique sticky ends. 0 kb 1 kb 2 kb B-7 kb PCR product B = BamHI E = EcoRI H = Hindill N = Notl S = Smal X = Xbal AmpR 5 kb S-5 kb The plasmid into which you want to clone the p53 gene is shown below. X 10 kb 8 kb 10 kb PCDNA3 plasmid 14 kb 11 kb E-0 bases N-20 bases S-30 bases H-40 bases .S-1 kb Your group is a part of a research team that just received a grant map of the p53 PCR product showing study cancer. As a first step, restriction enzyme cut sites that is mutant in many types of varivor vena. Below is a diagram of the DNA product of a PCR amplification of the region of the human genome containing p53. The white region represents the coding region of the gene. The letters above the DNA represent the locations of specific restriction enzyme cut sites. The numbers below each restriction site represent the number of kilobases (kb) from the first restriction site (at 0 kb). Each of the restriction enzymes creates unique sticky ends. 0 kb 1 kb 2 kb B-7 kb PCR product B = BamHI E = EcoRI H = Hindill N = Notl S = Smal X = Xbal AmpR 5 kb S-5 kb The plasmid into which you want to clone the p53 gene is shown below. X 10 kb 8 kb 10 kb PCDNA3 plasmid 14 kb 11 kb E-0 bases N-20 bases S-30 bases H-40 bases .S-1 kb