The rule of three states that the retention factor for a given solute decreases approximately threefold when

Question:

The "rule of three" states that the retention factor for a given solute decreases approximately threefold when the organic phase increases by 10%. In Figure 24-12, tm = 2.7 min. Find k for peak 5 at 50% B. Predict the retention time for peak 5 at 40% B and compare the observed and predicted times.