Question: Using the pKa data in Table 4.1 and the Henderson-Hasselbalch equa-tion, calculate the approximate net charge on each of the four common ribonucleoside 5 -monophosphates

Using the pKa data in Table 4.1 and the Henderson-Hasselbalch equa-tion, calculate the approximate net charge on each of the four common ribonucleoside 5€² -monophosphates (rNMPs) at pH 3.8. If a mixture of these (rNMPs) was placed in an electrophoresis apparatus, as shown, draw four bands to predict the direction and relative migration rate of each.

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