You have discovered a novel eukaryotic RNA polymerase To study this enzyme, you have developed an in vitro assay with an inhibitor of RNA chain elongation, 3 deoxy 5 32P CTP You are surprised by the fact that no 32P is found in the transcribed RNA What does this result tell you about the mechanism of the polymerase

Question: You have discovered a novel eukaryotic RNA polymerase. To study this enzyme, you have developed an in vitro assay with an inhibitor of RNA chain

You have discovered a novel eukaryotic RNA polymerase. To study this enzyme, you have developed an in vitro assay with an inhibitor of RNA chain elongation, 3′-deoxy-5′[α-32P] CTP. You are surprised by the fact that no 32P is found in the transcribed RNA! What does this result tell you about the mechanism of the polymerase?

Step by Step Solution

★★★★★

3.45 Rating (158 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

Normal RNA chain elongation requires a 3OH group Because the inhibitor lacks this functiona... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (1 attachment)

930-M-S-B-C (1764).docx

120 KBs Word File

Students Have Also Explored These Related Biochemistry Questions!

Patricia currently manages the polished chrome division of Nash Broadway, a business that specializes in ceiling light fixtures. Its performance has been stable for the past few years. However, the...

You have discovered a novel protein that has a pI = 5.5. To study the functional properties of this new protein, your research group has made a mutant that contains two amino acid changes-namely, a...

You have discovered a drug that inhibits the activity of inorganic pyrophosphatase. What effect would this drug have on DNA synthesis?

A gel retardation assay can be used to study the binding of proteins to a segment of DNA. In the experiment shown here, a gel retardation assay was used to examine the requirements for the binding of...

Famous musiclan and amateur blochemist Teddy Swims has discovered a novel weed killer called "Lose Control ( of your chloroplasts ) " . polson denatures the last enzyme in the photosys c . This...

. Required question. 25 points. John Miller, a research associate at Applegate State University, has discovered a novel process of extracting biodiesel out of genetically modified corn. The biodiesel...

a) A researcher recently discovered a novel dark green honey produced by a fluorescent-colored sting bee on Banggi Island in Sabah. Results of the nutritional analysis of the green honey are listed...

You have discovered a novel self-cleaving catalytic RNA that cleaves the phosphodiester linkage between B-1 and B+1. Here is a summary of some of the data you have collected: Rates of cleavage are...

You have discovered a novel toxin in snake venom and decide to name it VEN 1 . You think it might have therapeutic potential, and want to characterize its behavior. You solve the crystal structure,...

MATERIAL Company name - AMA Co. Ltd. Company address - 17 LINE 4-6, 199034, Saint-Petersburg, Russia Company description AMA Co. was founded in 1997 in Saint Petersburg, Russia, and has been...

Pharmaceutical manufacturer DEF recently discovered a novel drug for treating dementia, which it now has to submit to the FDA (Food and Drug Administration) for a long and tedious process of clinical...

How much federal income tax should Aman report if she earned taxable income of $32 920 and $17 700 from her two jobs? Use the 2012 federal income tax brackets and rates in Table 3.3 to answer the...

Owens parents, who are retired, have gifted Owen $2,500 every December since Ellies birth in 2015, which Owen then immediately contributed to an RESP for Ellie before year-end. i. For tax purposes,...

Explain fully whether it is better to use homelessness funding for more housing and therapeutic support rather than for WiFi.

The right-angle plate is formed from a flat plate having a mass p per unit area and is welded to the horizontal shaft mounted in the bearing at O. If the shaft is free to rotate, determine the...

On May 3, 2022, Eisler Company consigned 80 freezers, costing $500 each, to Remmers Company. The cost of shipping the freezers amounted to $840 and was paid by Eisler Company. On December 30, 2022, a...

A newly issued bond with 1 year to maturity has a price of 100, which equals its face value. The coupon rate on the bond is 15%; the probability of default in 1 year is 35%; and the bonds payoff in...

Describe the steps that should be followed in estimating cost functions.

Allee Corp. is evaluating a revenue arrangement to determine proper revenue recognition. The contract is for construction of 10 speedboats for a contract price of $400,000. The customer needs the...

Suppose 1% of a certain brand of Christmas lights is defective. Use the Poisson approximation to compute the probability that in a box of 25 there will be at most one defective bulb.

How does the difference between the b-chain and the g-chain of hemoglobin explain the differences in oxygen binding between Hb A and Hb F?

What is the effect of the altered amino acid sequence in Hb S that causes the cells to form sickle shapes?

Given the purpose of hemoglobin, what could one envision as a downside to having all our hemoglobin as Hb F instead of Hb A?

d. Calculating the price of each bond 1 year from now, the expected capital gains yield for each bond, and the expected total return for eanh hond Years to maturity V81 4 Expected CG Yield Expected...

Kuhn company is considering a new project that will require an initial investment of $20 million. It has a target capital structure of 35% debt, 2% preferred stock and 63% common equity. Kuhn has...

Daddi Mac, Inc., doesnt face any taxes and has $288.32 million in assets, currently financed entirely with equity. Equity is worth $34 per share, and book value of equity is equal to market value of...