You wish to isolate a large amount of Fab fragments in order to examine their binding to

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You wish to isolate a large amount of Fab fragments in order to examine their binding to protein X by X-ray crystallography. At first, you inject a large rabbit with protein X to obtain antibody. On further reflection, you decide to inject the antigen into a mouse in order to produce monoclonal antibodies.
(a) How can you purify protein X-specific antibodies from rabbit serum or the hybridoma medium?
(b) How do the rabbit and mouse antibody preparations differ?
(c) Would the rabbit or mouse antibodies be more suitable for X-ray crystallography?
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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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