Question: (a) Explain how to measure k and resolution. (b) State three methods for measuring tm in reversed-phase chromatography. (c) Estimate tm for a 0.46
(b) State three methods for measuring tm in reversed-phase chromatography.
(c) Estimate tm for a 0.46 × 15 cm column containing 5-μm particles operating at a flow rate of 1.5 mL/min. Estimate tm if the particle size were 3.5 μm instead.
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a To find k measure the retention time for the peak of interest t r and the ... View full answer
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