Question: A membrane glycoprotein-synthesizing system was devised that contained endoplasmic reticulum membranes and all the necessary ingredients for the in vitro synthesis of a protein. The

A membrane glycoprotein-synthesizing system was devised that contained endoplasmic reticulum membranes and all the necessary ingredients for the in vitro synthesis of a protein. The complete synthesis of the protein molecule required 40 minutes. When the detergent Triton X-100 was added within a few minutes after the initiation of polypeptide synthesis, the resulting protein was devoid of glycosyl groups.
(a) Explain the action of the detergent.
(b) Would the protein have been glycosylated if the detergent had been added 35 minutes after initiation of protein synthesis?

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