Question: A mixture of amino acids that do not separate sufficiently when a single technique is used can often be separated by two-dimensional chromatography. In this

A mixture of amino acids that do not separate sufficiently when a single technique is used can often be separated by two-dimensional chromatography. In this technique, the mixture of amino acids is applied to a piece of filter paper and separated by chromatographic techniques. The paper is then rotated 90°, and the amino acids are further separated by electrophoresis, producing a type of chromatogram called a fingerprint. Identify the spots in the fingerprint obtained from a mixture of Ser, Glu, Leu, His, Met, and Thr.

Chromatography

Step by Step Solution

★★★★★

3.38 Rating (157 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

Since the mixture of amino acids is in a solution of pH 5 His will have an overall positive ch... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (1 attachment)

948-C-O-C-R-O-A (2109).docx

120 KBs Word File

Students Have Also Explored These Related Organic Chemistry Questions!

Suppose that Mr. Dubinski has obtained from Blaine's banker the quotes below for default spreads over 10-year Treasury bonds (note that these differ from the more general corporate bonds yields in...

Pig liver esterase is an enzyme that catalyzes the hydrolysis of esters. It hydrolyzes esters of L-amino acids more rapidly than esters of D-amino acids. How can this enzyme be used to separate a...

In Figure part of a long insulated wire carrying current r = 5.78 mA is bent into a circular section of radius R = 1.89 cm. In unit-vector notation, what is the magnetic field at the center of...

A mixture of 15 amino acids gave the fingerprint shown in the below (see also Problem 42). Identify the spots. (Hint 1: Pro reacts with ninhydrin to form a yellow color; Phe and Tyr form a...

Paper chromatography was used to separate a mixture of amino acids. The mixture was run in two dimensions using two different solvents. The chromatogram obtained is shown below. a. Explain briefly...

Research Report Students name Professors name Institution Date Contents Title .. 3 Abstract 3 Acknowledgement .3 Introduction .4 Main body of Report/Methodology 5 Various kinds of isolation.6...

please help answer these "In this experiment, chromatography was used to analyze amino acids in solution. Standards were used to identify unknown amino acids in a mixture. Ascending layer...

CALCULATIONS 2. +/- R2 = Vterm,BSA +/- R2 = Vterm, lysozyme Vterm,BSA 3. = Vterm,lysozyme M = M 4. % diff = [ratioexp - ratioacc * 100 = * 100 = ratioacc Sratioexp = | ratioexp - ratio acc |= Compare...

Question 4 A biochemist want to separate a mixture of amino acids (Glutamate, Histidine, and Arginine) and decides to run to change throphy. This was the described in the FPG sum21 practical manual -...

Question 1 7 ( 1 . 5 points ) Which statement best summarizes the digestion of proteins? Proteins are denatured by stomach acid. Amine groups are removed from all amino acids. All peptide linkages...

Which statement best summarizes the digestion of proteins? Question 3 options: 1 ) Amino acids are combined to produce proteins or enzymes. 2 ) All peptide linkages are hydrolyzed to produce a...

Which of the following procedures is not part of those that are performed under a review of the financial statements of a private corporation? a. Interview with management to discuss possible...

Andrea bought futures contracts on 560,000 pounds of domestic sugar at a price of 21.48 cents per pound. Futures contracts on sugar are for 112,000 pounds. What is the amount of Andrea's profit or...

\ int x ^ ( 2 ) e ^ ( - 2 x ) dx

Jarriot, Inc., presented two years of data for its Furniture Division and its Houseware Division. Furniture Division: Houseware Division: Required: 1. Compute the ROI and the margin and turnover...

Gable Incorporated provides legal services. During 2015, the company provides services of $500,000 on account. Of this amount, $70,000 remains uncollected at the end of the year. An aging schedule as...

Consider the following situations that involve multiple objectives: a. Suppose you want to go out for dinner. What are your fundamental objectives? Create a fundamental-objectives hierarchy. b....

On January 1, 20Y3, The Simmons Group, Inc., purchased the assets of NWS Insurance Co. for $36,000,000, a price reflecting an $8,000,000 goodwill premium. On December 31, 20Y9, The Simmons Group...

On January 1, 2009, Affordable Financial, Inc., purchased the assets of Bisko Insurance Co. for $65,000,000, a price reflecting a $10,000,000 goodwill premium. On December 31, 2012, Affordable...

Briefly explain whether you agree with the following statement: International trade is more important to the U.S. economy than to most other economies.

Apply Hammond's postulate to decide which reaction is faster: addition of HBr to 2 methylpropene or addition of HBr to trans-2-butene. Assume that the energy difference between the starting alkenes...

How many alkenes containing one double bond can react with H, over a Pt/C catalyst to give methylcyelopentane? Give their structures.

The alkene 3,3-dimethyl-1 -butene undergoes acid-catalyzed hydration with rearrangement. Use the mechanism of hydration and rearrangement to predict the structure of the hydration product of this...

Pension funds pay lifetime annuities to recipients. If a firm will remain in business indefinitely, the pension obligation will resemble a perpetuity. Suppose, therefore, that you are managing a...

Our new computer system cost us $106,000. We will outgrow it in five years. When we sell it, we will probably get only 20% of the purchase price. CCA on the computer will be calculated at a 30% rate...

(8 pts) Find these critical values: a) t(25, 0.05) b) t(40, 0.975)