A mutant form of polypeptide hormone angiotensin II has the amino acid composition

The following observations are made:
€¢ Trypsin yields a dipeptide containing Asp and Arg, and a hexapeptide with all the rest. €¢ Cyanogen bromide cleavage yields a dipeptide containing Phe and Pro, and a hexapeptide containing all the others.
€¢ Chymotrypsin cleaves the hormone into two tetrapeptides, of composition

€¢ The dipeptide of composition (Pro, Phe) cannot be cleaved by either chymotrypsin or carboxypeptidase.
What is the sequence of angiotensin II?

Transcribed Image Text:

CAsp Ang, le, Met, the, Pro, Tyr, Vd) Asp, Arg, Tyr, Va) and (e, Met,Phe,Prol