Question: A sample of chromatin was partially digested by the enzyme Staphylococcal nuclease. The DNA fragments from this digestion were puriï¬ed and resolved on a polyacrylamide

A sample of chromatin was partially digested by the enzyme Staphylococcal nuclease. The DNA fragments from this digestion were puriï¬ed and resolved on a polyacrylamide gel. A set of DNA restriction fragments was used as markers. Distances of migration (d) are given below in cm. From these data, estimate the nucleosome repeat distance in the chromatin.

Marker DNA Fragmen Chrm atin DNA Fragment Size (bp) d (cm) Cm 94 145 263 498 794 40 34.2 25.2 16.7 11.5 30 19.2 14.4 11.5

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