Consider the l bacteriophage DNA molecule as shown in Figure 21.15. Total digestion with the two restriction endonucleases used does not allow unambiguous placement of restriction sites, as shown in the map. Describe restriction patterns-either from partial digestion or from digestion with both Bam HI and Eco RI-that would help to establish the specific map of restriction sites shown in Figure 21.15(b).
a.

b.

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Fragment Fragmn EccR Bam H digest digest Fragment Fragment size, kb size, kb 21.2 16.8 12.1 FIA ET C' 5.8 5.6 4.9 7.2 6.8 6.5 5.6 b.b D, F A, C Fragments with very similar sizes fonm b one band on a gel. BarrHl sites λ DNA BartHll segments A EcoRl sites λ DNA EcoRl segments E F 48.5 DNA length, kb