RFLPs can include sequence changes created by single-base change, single-base insertion, single-base deletion, or tetranucleotide repeat, as

Question:

RFLPs can include sequence changes created by single-base change, single-base insertion, single-base deletion, or tetranucleotide repeat, as shown in Figure 21.21. For each polymorphism, show a speciï¬c sequence change that would alter the restriction fragmentation pattern at that site and describe how the pattern would change-that is, addition or loss of a site for a speciï¬c restriction endonuclease. Refer to Table 21.3 for the cleavage sites of several common restriction nucleases.