Explain how the following affect the Tm of double-stranded DNA: (a) Increasing the monovalent salt concetration. (b)

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Explain how the following affect the Tm of double-stranded DNA:
(a) Increasing the monovalent salt concetration.
(b) Decreasing the pH.
(c) Increasing the pH.
(d) Increasing the concentration of formamide?

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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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Question Posted: Apr 30, 2016 02:53 PM

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Explain how the following affect the Tm of double-stranded DNA: (a) Increasing the monovalent salt concetration. (b)

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a Monovalent salts increase the T m because they attenuate the repulsions between the negatively cha...View the full answer

Fundamentals of biochemistry Life at the Molecular Level

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