Question: In the Western blot shown here, proteins were isolated from red blood cells and muscle cells from two different individuals. One individual was unaffected, and
In the Western blot shown here, proteins were isolated from red blood cells and muscle cells from two different individuals. One individual was unaffected, and the other individual suffered from a disease known as thalassemia, which involves a defect in hemoglobin. In the Western blot, the gel blot was exposed to an antibody that recognizes (3 globin, which is one of the polypeptides that constitute hemoglobin. Equal amounts of total cellular proteins were added to each lane.
Lane 1: Proteins isolated from normal red blood cells
Lane 2: Proteins isolated from the red blood cells of a thalassemia patient
Lane 3: Proteins isolated from normal muscle cells
Lane 4: Proteins isolated from the muscle cells of a thalassemia patient
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Explain these results.
2 3 4
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Lane 1 shows that globin is made in normal red blood cells In ... View full answer
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