Let's suppose a researcher was interested in the effects of mutations on the expression of a protein-encoding gene for a protein that is 472 amino acids in length. This protein is expressed in leaf cells of Arabidopsis thaliana. Because the average molecular mass of an amino acid is 120 Da, this protein has a molecular mass of approximately 56,640 Da. Make a drawing that shows the expected results of a Western blot using proteins isolated from the leaf cells that were obtained from the following individuals:
Lane 1. A plant homozygous for a nonmutant gene
Lane 2. A plant homozygous for a deletion that removes the promoter for this gene
Lane 3. A heterozygous plant in which one gene is nonmutant and the other gene has a mutation that introduces an early stop codon at codon 112
Lane 4. A plant homozygous for a mutation that introduces an early stop codon at codon 112
Lane 5. A plant homozygous for a mutation that changes codon 108 from a phenylalanine codon into a leucine codon