Question: Reticulocyte (immature red blood cell) lysates can be made devoid of mRNA by ribonuclease treatment followed by inactivation and removal of the ribonuclease. These lysates

Reticulocyte (immature red blood cell) lysates can be made devoid of mRNA by ribonuclease treatment followed by inactivation and removal of the ribonuclease. These lysates are now capable of synthesizing protein when mRNA, GTP, and aminoacyl-tRNAs are added. How could you use this system to demonstrate that a protein is synthesized from the N-terminus to the C-terminus?

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