An article in Applied Biochemistry and Biotechnology (A Statistical Approach for Optimization of Polyhydroxybutyrate Production by Bacillus

An article in Applied Biochemistry and Biotechnology (€œA Statistical Approach for Optimization of Polyhydroxybutyrate Production by Bacillus sphaericus NCIM 5149 under Submerged Fermentation Using Central Composite Design€ (2010, Vol. 162(4), pp. 996€“1007)] described an experiment to optimize the production of polyhydroxybutyrate (PHB). Inoculum age, pH, and substrate were selected as factors, and a central composite design was conducted. Data follow.

(a) Plot the points at which the experimental runs were made. What type of design is used?

(b) Fit a second-order response surface model to the data.

(c) Does a residual analysis indicate any problems?

(d) Construct a contour plot and response surface for PHB amount in terms of two factors.

(e) Can you recommend values for inoculum age, pH and substrate to maximize production?

Transcribed Image Text:

PHB PHB Inoculum age Substrate (g/L) 1 1 2.5 (g/L) Run (h) 12 24 pH 4 1 0.84 0.55 1.96 8. 6. 18 2.